Generator
Part:BBa_K861073
Designed by: Kuanwei Sheng Group: iGEM12_WHU-China (2012-09-21)
Constitutively expressed AdrA
AdrA contains a GGDEF domain that can catalyze GTP into c-di-GMP. c-di-GMP is a magic molecule that can activate bcsB gene, a major component of cellulose synthetase complex. Also, c-di-GMP can serve as a second messager to inhibit motility and increse biofilm formation and adhesion of bacteria. This part was designed to characterize the effect of the gene AdrA on cell colonization.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 589
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
n/a | Constitutively expressed AdrA |